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SNX12 Polyclonal antibody

SNX12 Polyclonal Antibody for IF-Fro, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-Fro, WB, ELISA

Conjugate

Unconjugated

Cat No : 12374-1-AP

Print datasheet

Synonyms

SNX12, sorting nexin 12

验证数据展示

  • WB analysis using 12374-1-AP

    WB analysis using 12374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12374-1-AP (SNX12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12374-1-AP

    IF Staining of HeLa using 12374-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (12374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12374-1-AP targets SNX12 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-Fro, WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNX12 fusion protein Ag3014 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting nexin 12
Synonyms SNX12, sorting nexin 12
Calculated Molecular Weight 172 aa, 20 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC020559
Gene Symbol SNX12
Gene ID (NCBI) 29934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).

实验方案

Product Specific Protocols
WB protocol for SNX12 antibody 12374-1-APDownload protocol
IF protocol for SNX12 antibody 12374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols