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SNX15 Polyclonal antibody

SNX15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16049-1-AP

Print datasheet

Synonyms

Sorting nexin-15, sorting nexin 15, HSAF001435

验证数据展示

  • WB analysis using 16049-1-AP

    WB analysis using 16049-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 16049-1-AP

    IHC staining of mouse kidney using 16049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 16049-1-AP

    IHC staining of mouse kidney using 16049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, human testis tissue, SH-SY5Y cells, mouse brain tissue, mouse lung tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16049-1-AP targets SNX15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNX15 fusion protein Ag9006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting nexin 15
Synonyms Sorting nexin-15, sorting nexin 15, HSAF001435
Calculated Molecular Weight 342 aa, 38 kDa
Observed Molecular Weight 50 kDa and 38 kDa
GenBank Accession NumberBC009897
Gene Symbol SNX15
Gene ID (NCBI) 29907
RRIDAB_2192367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

实验方案

Product Specific Protocols
WB protocol for SNX15 antibody 16049-1-APDownload protocol
IHC protocol for SNX15 antibody 16049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols