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验证数据展示

  • WB analysis using 68239-1-Ig

    WB analysis using 68239-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68239-1-Ig (SNX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human kidney using 68239-1-Ig

    IHC staining of human kidney using 68239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 68239-1-Ig

    IHC staining of human kidney using 68239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 68239-1-Ig

    IF Staining of U2OS using 68239-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SNX15 antibody (68239-1-Ig, Clone: 1A10B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

SNX15 Monoclonal antibody

SNX15 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68239-1-Ig

产品说明书

CloneNo. 1A10B7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Sorting nexin-15, sorting nexin 15, HSAF001435, 1A10B7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68239-1-Ig targets SNX15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SNX15 fusion protein Ag8839 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting nexin 15
别名 Sorting nexin-15, sorting nexin 15, HSAF001435, 1A10B7
计算分子量 342 aa, 38 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC009897
基因名称 SNX15
Gene ID (NCBI) 29907
RRIDAB_2935326
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

实验方案

Product Specific Protocols
WB protocol for SNX15 antibody 68239-1-IgDownload protocol
IHC protocol for SNX15 antibody 68239-1-IgDownload protocol
IF protocol for SNX15 antibody 68239-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols