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验证数据展示

  • WB analysis using 13492-1-AP

    WB analysis using 13492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13492-1-AP (SNX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HT-29 using 13492-1-AP

    IF Staining of HT-29 using 13492-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SNX16 antibody (13492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

SNX16 Polyclonal antibody

SNX16 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13492-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Sorting nexin-16, sorting nexin 16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells, U-251 cells
Positive IF/ICC detected inHT-29 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13492-1-AP targets SNX16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SNX16 fusion protein Ag4315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 16
别名 Sorting nexin-16, sorting nexin 16
计算分子量 344 aa, 39 kDa
观测分子量39-50 kDa
GenBank蛋白编号BC033630
基因名称 SNX16
Gene ID (NCBI) 64089
RRIDAB_3085416
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57768
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX16,Sorting nexin 16, is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which mediates membrane association by interaction with phosphoinositides. SNX16 associates with late endosome membranes as is involved in tubule formation, cholesterol transport, and transport of tetraspanin CD81. It also inhibits cell migration and tumorigenesis. SNX16 directs the sorting of EGFR to the endosomal compartment and thus regulates EGF-induced cell signaling (PMID:15292396). It may function in the trafficking of proteins between the early and late endosomal compartments. SNX16 was detected 49-53 kDa in human (PMID:12813048).

实验方案

Product Specific Protocols
WB protocol for SNX16 antibody 13492-1-APDownload protocol
IF protocol for SNX16 antibody 13492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols