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SNX16 Polyclonal antibody

SNX16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13492-1-AP

Print datasheet

Synonyms

Sorting nexin-16, sorting nexin 16

验证数据展示

  • WB analysis using 13492-1-AP

    WB analysis using 13492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13492-1-AP (SNX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HT-29 using 13492-1-AP

    IF Staining of HT-29 using 13492-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SNX16 antibody (13492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, Raji cells, U-251 cells
Positive IF/ICC detected inHT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13492-1-AP targets SNX16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SNX16 fusion protein Ag4315 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting nexin 16
Synonyms Sorting nexin-16, sorting nexin 16
Calculated Molecular Weight 344 aa, 39 kDa
Observed Molecular Weight39-50 kDa
GenBank Accession NumberBC033630
Gene Symbol SNX16
Gene ID (NCBI) 64089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX16,Sorting nexin 16, is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which mediates membrane association by interaction with phosphoinositides. SNX16 associates with late endosome membranes as is involved in tubule formation, cholesterol transport, and transport of tetraspanin CD81. It also inhibits cell migration and tumorigenesis. SNX16 directs the sorting of EGFR to the endosomal compartment and thus regulates EGF-induced cell signaling (PMID:15292396). It may function in the trafficking of proteins between the early and late endosomal compartments. SNX16 was detected 49-53 kDa in human (PMID:12813048).

实验方案

Product Specific Protocols
WB protocol for SNX16 antibody 13492-1-APDownload protocol
IF protocol for SNX16 antibody 13492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols