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验证数据展示

  • WB analysis using 68256-1-Ig

    WB analysis using 68256-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68256-1-Ig

    IF Staining of HepG2 using 68256-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

SNX17 Monoclonal antibody

SNX17 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68256-1-Ig

产品说明书

CloneNo. 3B8F6

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, pig

应用

WB, IF/ICC, ELISA

别名

KIAA0064, SNX17, sorting nexin 17

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, pig brain tissue, Jurkat cells, rat brain tissue, HeLa cells
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68256-1-Ig targets SNX17 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat, pig
免疫原 SNX17 fusion protein Ag32624 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting nexin 17
别名 KIAA0064, SNX17, sorting nexin 17
计算分子量 53 kDa
观测分子量 55 kDa
GenBank蛋白编号BC002524
基因名称 SNX17
Gene ID (NCBI) 9784
RRIDAB_2935341
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15036
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

实验方案

Product Specific Protocols
WB protocol for SNX17 antibody 68256-1-IgDownload protocol
IF protocol for SNX17 antibody 68256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols