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验证数据展示

  • WB analysis using 84215-4-RR

    WB analysis using 84215-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84215-4-RR

    IF Staining of MCF-7 using 84215-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SNX18 antibody (84215-4-RR, Clone: 241537D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84215-4-RR

    Affinity and Kinetic Characterization of 84215-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84215-4-RR against Human SNX18 were performed. The affinity constant is 0.378 nM.

SNX18 Recombinant antibody

SNX18 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84215-4-RR

产品说明书

CloneNo. 241537D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Sorting nexin-18, SH3PXD3B, SH3 and PX domain-containing protein 3B, 241537D11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84215-4-RR targets SNX18 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SNX18 fusion protein Ag16645 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sorting nexin 18
别名 Sorting nexin-18, SH3PXD3B, SH3 and PX domain-containing protein 3B, 241537D11
计算分子量 628 aa, 69 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC060791
基因名称 SNX18
Gene ID (NCBI) 112574
RRIDAB_3671771
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96RF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

实验方案

Product Specific Protocols
WB protocol for SNX18 antibody 84215-4-RRDownload protocol
IF protocol for SNX18 antibody 84215-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols