• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SNX20 Polyclonal antibody

SNX20 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13180-1-AP

Print datasheet

Synonyms

SLIC 1, SLIC1, SNX20, sorting nexin 20

验证数据展示

  • WB analysis of COLO 320 using 13180-1-AP

    WB analysis of COLO 320 using 13180-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13180-1-AP (SNX20 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13180-1-AP

    IHC staining of human placenta using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 13180-1-AP

    IHC staining of human placenta using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of COLO 320 using 13180-1-AP

    IP experiment of COLO 320 using 13180-1-AP

    IP result of anti-SNX20 (IP:13180-1-AP, 4ug; Detection:13180-1-AP 1:300) with COLO 320 cells lysate 2000ug.

经过测试的应用

Positive WB detected inCOLO 320 cells, mouse thymus tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman placenta tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13180-1-AP targets SNX20 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNX20 fusion protein Ag3847 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting nexin 20
Synonyms SLIC 1, SLIC1, SNX20, sorting nexin 20
Calculated Molecular Weight 316 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC027944
Gene Symbol SNX20
Gene ID (NCBI) 124460
RRIDAB_10638911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z614
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNX20 antibody 13180-1-APDownload protocol
IHC protocol for SNX20 antibody 13180-1-APDownload protocol
IP protocol for SNX20 antibody 13180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols