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SNX25 Polyclonal antibody
SNX25 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | EL-4 cells, Raji cells, Jurkat cells, mouse thymus tissue, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | Raji cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13294-1-AP targets SNX25 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SNX25 fusion protein Ag3995 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sorting nexin 25 |
| Synonyms | Sorting nexin-25, sorting nexin 25, SBBI31, MSTP043 |
| Calculated Molecular Weight | 98 kDa |
| Observed Molecular Weight | 98-105 kDa, 65 kDa |
| GenBank Accession Number | BC029868 |
| Gene Symbol | SNX25 |
| Gene ID (NCBI) | 83891 |
| RRID | AB_2192549 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3E2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-β receptors. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation.This antibody can recognize two isoforms: 98-105 kDa and 65 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX25 antibody 13294-1-AP | Download protocol |
| IF protocol for SNX25 antibody 13294-1-AP | Download protocol |
| IP protocol for SNX25 antibody 13294-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Struct Funct Neural expression of sorting nexin 25 and its regulation of tyrosine receptor kinase B trafficking. | ||
PLoS One SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.
| ||
Neurosci Lett Circadian rhythms of sorting nexin 25 in the mouse suprachiasmatic nucleus. | ||
Nat Immunol Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway | ||
Redox Biol Redox-modulated SNX25 as a novel regulator of GPCR-G protein signaling from endosomes |
