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验证数据展示

  • WB analysis using 68292-1-Ig

    WB analysis using 68292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68292-1-Ig (SNX3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68292-1-Ig

    WB analysis of HeLa using 68292-1-Ig

    WB result of SNX3 antibody (68292-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX3 transfected HeLa cells.

  • IF Staining of A431 using 68292-1-Ig

    IF Staining of A431 using 68292-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX3 antibody (68292-1-Ig, Clone: 2B5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • KD/KO验证

SNX3 Monoclonal antibody

SNX3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68292-1-Ig

产品说明书

CloneNo. 2B5B7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

2B5B7, Grd19, MCOPS8, Protein SDP3, SDP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68292-1-Ig targets SNX3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1160

Product name: Recombinant human SNX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-162 aa of BC008444

Sequence: MAETVADTRRLITKPQNLNDAYGPPSNFLEIDVSNPQTVGVGRGRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSELERESKVVVPPLPGKAFLRQLPFRGDDGIFDDNFIEERKQGLEQFINKVAGHPLAQNERCLHMFLQDEIIDKSYTPSKIRHA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting nexin 3
别名 2B5B7, Grd19, MCOPS8, Protein SDP3, SDP3
计算分子量 19 kDa
观测分子量16-20 kDa
GenBank蛋白编号BC008444
基因名称 SNX3
Gene ID (NCBI) 8724
RRIDAB_2935372
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO60493
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

实验方案

Product Specific Protocols
IF protocol for SNX3 antibody 68292-1-IgDownload protocol
WB protocol for SNX3 antibody 68292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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