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验证数据展示

  • WB analysis of HeLa using 25763-1-AP

    WB analysis of HeLa using 25763-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25763-1-AP (SNX32 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25763-1-AP

    IHC staining of mouse brain using 25763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25763-1-AP

    IHC staining of mouse brain using 25763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SNX32 Polyclonal antibody

SNX32 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25763-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (3)

别名

SNX32, SNX6B, sorting nexin 32, Sorting nexin 6B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 900 μg/ml

库存：现货

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25763-1-AP targets SNX32 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP, IF, CoIP
经测试反应性 human, mouse
文献引用反应性human, pig
免疫原 SNX32 fusion protein Ag22653 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 32
别名 SNX32, SNX6B, sorting nexin 32, Sorting nexin 6B
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC040981
基因名称 SNX32
Gene ID (NCBI) 254122
RRIDAB_2880229
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XE0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX32 (Sorting nexin-32, also known as SNX6B) may be involved in several stages of intracellular trafficking.

实验方案

Product Specific Protocols
WB protocol for SNX32 antibody 25763-1-APDownload protocol
IHC protocol for SNX32 antibody 25763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,pigCoIP,WB,IF,IP

J Virol

SNX32 is a host restriction factor that degrades African swine fever virus CP204L via the RAB1B-dependent autophagy pathway

Authors - Wenping Yang
  • KD Validated
View Article