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SNX4 Polyclonal antibody

SNX4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11506-1-AP

Print datasheet

Synonyms

Sorting nexin-4, sorting nexin 4

验证数据展示

  • WB analysis using 11506-1-AP

    WB analysis using 11506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11506-1-AP (SNX4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 11506-1-AP

    WB analysis of A549 using 11506-1-AP

    WB result of SNX4 antibody (11506-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX4 transfected A549 cells.

  • IHC staining of human stomach using 11506-1-AP

    IHC staining of human stomach using 11506-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11506-1-AP (SNX4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, A431 cells, A549 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11506-1-AP targets SNX4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SNX4 fusion protein Ag2058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting nexin 4
Synonyms Sorting nexin-4, sorting nexin 4
Calculated Molecular Weight 450 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC018762
Gene Symbol SNX4
Gene ID (NCBI) 8723
RRIDAB_2286398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNX4 antibody 11506-1-APDownload protocol
IHC protocol for SNX4 antibody 11506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Authors - Chuchu Zhou
View Article