验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse kidney tissue, human kidney tissue, Neuro-2a cells, K-562 cells, Jurkat cells, U2OS cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17918-1-AP targets SNX5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SNX5 fusion protein Ag12330 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorting nexin 5 |
| 别名 | Sorting nexin-5 |
| 计算分子量 | 404 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC093623 |
| 基因名称 | SNX5 |
| Gene ID (NCBI) | 27131 |
| RRID | AB_2192708 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5X3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX5 antibody 17918-1-AP | Download protocol |
| IHC protocol for SNX5 antibody 17918-1-AP | Download protocol |
| IP protocol for SNX5 antibody 17918-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
Nat Commun SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion. | ||
J Cell Biol Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
| ||
Oxid Med Cell Longev Sorting Nexin 5 Plays an Important Role in Promoting Ferroptosis in Parkinson's Disease.
| ||
Cells Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells |
