验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, RAW 264.7 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse brain tissue, human prostate cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
10114-1-AP targets SNX6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SNX6 fusion protein Ag0168 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorting nexin 6 |
| 别名 | TRAF4-associated factor 2, Sorting nexin-6, N-terminally processed, Sorting nexin-6 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC001798 |
| 基因名称 | SNX6 |
| Gene ID (NCBI) | 58533 |
| RRID | AB_2192720 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNH7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX6 antibody 10114-1-AP | Download protocol |
| IHC protocol for SNX6 antibody 10114-1-AP | Download protocol |
| IF protocol for SNX6 antibody 10114-1-AP | Download protocol |
| IP protocol for SNX6 antibody 10114-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Recycling of autophagosomal components from autolysosomes by the recycler complex. | ||
Acta Biochim Biophys Sin (Shanghai) SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.
| ||
Front Immunol Zika virus modulates mitochondrial dynamics, mitophagy, and mitochondria-derived vesicles to facilitate viral replication in trophoblast cells |
