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验证数据展示

  • WB analysis using 11494-1-AP

    WB analysis using 11494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11494-1-AP (SNX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 11494-1-AP

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 11494-1-AP

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNX8 Polyclonal antibody

SNX8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11494-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Mvp1, SNX8, sorting nexin 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11494-1-AP targets SNX8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNX8 fusion protein Ag2055 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 8
别名 Mvp1, SNX8, sorting nexin 8
计算分子量 465 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC021565
基因名称 SNX8
Gene ID (NCBI) 29886
RRIDAB_2923557
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5X2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexin 8 (SNX8) is a member of the sorting nexin family. Members of the sorting nexin family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX8 plays a role in the endosome-to-Golgi transport (PMID: 19782049). SNX8 can be used as a potential target for treating lysosomal storage disorder (PMID: 38519472).

实验方案

Product Specific Protocols
WB protocol for SNX8 antibody 11494-1-APDownload protocol
IHC protocol for SNX8 antibody 11494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols