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SNX9 Monoclonal antibody, PBS Only

SNX9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11C9

Cat no : 68255-1-PBS

Print datasheet

Synonyms

MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP

验证数据展示

  • WB analysis using 68255-1-Ig (same clone as 68255-1-PBS)

    WB analysis using 68255-1-Ig (same clone as 68255-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68255-1-Ig (SNX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68255-1-Ig (same clone as 68255-1-PBS)

    IF Staining of A431 using 68255-1-Ig (same clone as 68255-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SNX9 antibody (68255-1-Ig, Clone: 1B11C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

产品信息

68255-1-PBS targets SNX9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SNX9 fusion protein Ag8446 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sorting nexin 9
Synonyms MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP
Calculated Molecular Weight 595 aa, 67 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC005022
Gene Symbol SNX9
Gene ID (NCBI) 51429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5X1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).