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验证数据展示

  • WB analysis using 13202-1-AP

    WB analysis using 13202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13202-1-AP (SOAT1/ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SOAT1/ACAT1 Polyclonal antibody

SOAT1/ACAT1 Polyclonal Antibody for WB, ELISA
Cat No. 13202-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ACACT, ACACT1, ACAT, ACAT 1, ACAT1, Cholesterol acyltransferase 1, RP11 215I23.2, SOAT, SOAT1, STAT, sterol O acyltransferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HuH-7 cells, SW480 cells, THP-1 cells, mouse liver tissue, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13202-1-AP targets SOAT1/ACAT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SOAT1/ACAT1 fusion protein Ag3944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterol O-acyltransferase 1
别名 ACACT, ACACT1, ACAT, ACAT 1, ACAT1, Cholesterol acyltransferase 1, RP11 215I23.2, SOAT, SOAT1, STAT, sterol O acyltransferase 1
计算分子量 550 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC028940
基因名称 SOAT1/ACAT1
Gene ID (NCBI) 6646
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35610
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sterol O-acyltransferase 1 (SOAT1/ACAT1) catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed:16154994,16647063, 32433614, 32944968). It plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed:16154994, 9020103). SOAT1 has three isoforms with molecular weights of 65, 58, and 57 kDa.

实验方案

Product Specific Protocols
WB protocol for SOAT1/ACAT1 antibody 13202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols