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SOAT2 Polyclonal antibody

SOAT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21852-1-AP

Print datasheet

Synonyms

EC:2.3.1.26, ARGP2, Acyl-coenzyme A:cholesterol acyltransferase 2, ACAT-2, ACAT2

验证数据展示

  • WB analysis using 21852-1-AP

    WB analysis using 21852-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21852-1-AP

    IHC staining of human liver using 21852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 21852-1-AP

    IF Staining of HeLa using 21852-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

21852-1-AP targets SOAT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SOAT2 fusion protein Ag16364 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterol O-acyltransferase 2
Synonyms EC:2.3.1.26, ARGP2, Acyl-coenzyme A:cholesterol acyltransferase 2, ACAT-2, ACAT2
Calculated Molecular Weight 522 aa, 60 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC096090
Gene Symbol SOAT2
Gene ID (NCBI) 8435
RRIDAB_11183043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOAT2, also named as ACAT2, belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. SOAT2 is expressed preferentially in enterocytes of the intestine and hepatocytes of the liver. The role of SOAT2 in generating esterified cholesterol (EC) that is incorporated directly into atherogenic lipoproteins has made it an attractive target in dyslipidemia management and prevention. (PMID: 29246491)

实验方案

Product Specific Protocols
WB protocol for SOAT2 antibody 21852-1-APDownload protocol
IHC protocol for SOAT2 antibody 21852-1-APDownload protocol
IF protocol for SOAT2 antibody 21852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Death Discov

Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.

Authors - Huanji Xu
View Article