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验证数据展示

  • WB analysis using 27343-1-AP

    WB analysis using 27343-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27343-1-AP (SOCS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 27343-1-AP

    IP experiment of human placenta using 27343-1-AP

    IP result of anti-SOCS6 (IP:27343-1-AP, 4ug; Detection:27343-1-AP 1:500) with human placenta tissue lysate 1160 ug.

  • IHC staining of human colon using 27343-1-AP

    IHC staining of human colon using 27343-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 27343-1-AP

    IHC staining of human colon using 27343-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 27343-1-AP

    IHC staining of mouse colon using 27343-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SOCS6 Polyclonal antibody

SOCS6 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27343-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

SOCS 4, HSPC060, Cytokine-inducible SH2 protein 4, CIS-4, CIS4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Calu-1 cells, NIH/3T3 cells, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27343-1-AP targets SOCS6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 SOCS6 fusion protein Ag25594 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppressor of cytokine signaling 6
别名 SOCS 4, HSPC060, Cytokine-inducible SH2 protein 4, CIS-4, CIS4
计算分子量 60 kDa
观测分子量 60-75 kDa
GenBank蛋白编号BC020082
基因名称 SOCS6
Gene ID (NCBI) 9306
RRIDAB_3669595
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOCS6 (Suppressor of cytokine signaling 6) is also named as CIS4 and SOCS4. SOCS6 is one of eight members of the SOCS family of proteins. SOCS6 is involved in negative regulation of receptor signaling by increasing degradation mediated by ubiquitination of receptors or substrate proteins and induces apoptosis by targeting mitochondrial proteins (PMID: 25172101). SOCS6 is widely expressed in many tissues and is found to be downregulated in many cancers including colorectal cancer, gastric cancer, lung cancer, ovarian cancer, stomach cancer, thyroid cancer, hepatocellular carcinoma, and pancreatic cancer (PMID: 25172101). In addition to its molecular weight of 60 kDa, SOCS6 has a 70 kDa isoform (PMID: 34169835, PMID: 22125600).

实验方案

Product Specific Protocols
WB protocol for SOCS6 antibody 27343-1-APDownload protocol
IHC protocol for SOCS6 antibody 27343-1-APDownload protocol
IP protocol for SOCS6 antibody 27343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols