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SOCS6 Polyclonal antibody

SOCS6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27343-1-AP

Print datasheet

Synonyms

CIS 4, CIS4, HSPC060, SOCS 4, SOCS 6, SOCS4, SOCS6, SSI4, STAI4, STATI4

验证数据展示

  • IP experiment of human placenta using 27343-1-AP

    IP experiment of human placenta using 27343-1-AP

    IP result of anti-SOCS6 (IP:27343-1-AP, 4ug; Detection:27343-1-AP 1:500) with human placenta tissue lysate 1160 ug.

  • IHC staining of human colon using 27343-1-AP

    IHC staining of human colon using 27343-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 27343-1-AP

    IHC staining of human colon using 27343-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 27343-1-AP

    IHC staining of mouse colon using 27343-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27343-1-AP

    WB analysis using 27343-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27343-1-AP (SOCS6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27343-1-AP targets SOCS6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SOCS6 fusion protein Ag25594 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppressor of cytokine signaling 6
Synonyms CIS 4, CIS4, HSPC060, SOCS 4, SOCS 6, SOCS4, SOCS6, SSI4, STAI4, STATI4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-75 kDa
GenBank Accession NumberBC020082
Gene Symbol SOCS6
Gene ID (NCBI) 9306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOCS6 (Suppressor of cytokine signaling 6) is also named as CIS4 and SOCS4. SOCS6 is one of eight members of the SOCS family of proteins. SOCS6 is involved in negative regulation of receptor signaling by increasing degradation mediated by ubiquitination of receptors or substrate proteins and induces apoptosis by targeting mitochondrial proteins (PMID: 25172101). SOCS6 is widely expressed in many tissues and is found to be downregulated in many cancers including colorectal cancer, gastric cancer, lung cancer, ovarian cancer, stomach cancer, thyroid cancer, hepatocellular carcinoma, and pancreatic cancer (PMID: 25172101). In addition to its molecular weight of 60 kDa, SOCS6 has a 70 kDa isoform (PMID: 34169835, PMID: 22125600).

实验方案

Product Specific Protocols
WB protocol for SOCS6 antibody 27343-1-APDownload protocol
IHC protocol for SOCS6 antibody 27343-1-APDownload protocol
IP protocol for SOCS6 antibody 27343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols