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SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31400-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 31400-1-AP

    WB analysis using 31400-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31400-1-AP (SOD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, mouse liver tissue, rat liver tissue
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31400-1-AP targets SOD1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name superoxide dismutase 1, soluble
Synonyms
Calculated Molecular Weight16kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberNM_000454.5
Gene Symbol SOD1
Gene ID (NCBI) 6647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP00441
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
WB protocol for SOD1 antibody 31400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols