验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83857-6-RR targets SOD1 in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | superoxide dismutase 1, soluble |
Synonyms | IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1 |
Calculated Molecular Weight | 16kDa |
Observed Molecular Weight | 16-20 kDa |
GenBank Accession Number | NM_000454.5 |
Gene Symbol | SOD1 |
Gene ID (NCBI) | 6647 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P00441 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.
实验方案
Product Specific Protocols | |
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WB protocol for SOD1 antibody 83857-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |