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验证数据展示

  • WB analysis using 83857-6-RR

    WB analysis using 83857-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83857-6-RR (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83857-6-RR

    Affinity and Kinetic Characterization of 83857-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83857-6-RR against Human SOD1 were performed. The affinity constant is 13.0 nM.

SOD1 Recombinant antibody

SOD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83857-6-RR
CloneNo. 240888B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83857-6-RR targets SOD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name superoxide dismutase 1, soluble
Synonyms IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1
Calculated Molecular Weight16kDa
Observed Molecular Weight16-20 kDa
GenBank Accession NumberNM_000454.5
Gene Symbol SOD1
Gene ID (NCBI) 6647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00441
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
WB protocol for SOD1 antibody 83857-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols