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SOD1 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2b

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.098-100 ng/mL

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP50052-1

Print datasheet

Synonyms

ALS, ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]

Validation Data Gallery

  • Sample test of MP50052-1, SOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67480-2-PBS. Detection antibody: 67480-3-PBS. Cell lysate(30μg/well): HEK293, Jurkat, K-562, HSC-T6. Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

    Sample test of MP50052-1

    Sample test of MP50052-1, SOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67480-2-PBS. Detection antibody: 67480-3-PBS. Cell lysate(30μg/well): HEK293, Jurkat, K-562, HSC-T6. Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

  • Sandwich ELISA standard curve of MP50052-1, SOD1 Monoclonal Matched Antibody Pair - PBS only. 67480-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag28553. 67480-3-PBS was biotinylated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP50052-1

    Sandwich ELISA standard curve of MP50052-1, SOD1 Monoclonal Matched Antibody Pair - PBS only. 67480-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag28553. 67480-3-PBS was biotinylated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Cytometric bead array standard curve of MP50052-1, SOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67480-2-PBS. Detection antibody: 67480-3-PBS. Standard:Ag28553. Range: 0.098-100 ng/mL.

    Cytometric bead array standard curve of MP50052-1

    Cytometric bead array standard curve of MP50052-1, SOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67480-2-PBS. Detection antibody: 67480-3-PBS. Standard:Ag28553. Range: 0.098-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP50052-1 targets SOD1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.098-100 ng/mL (Cytometric Bead Array); 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: SOD1 Monoclonal antibody, PBS Only (Capture) 67480-2-PBS (5H6G2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: SOD1 Monoclonal antibody, PBS Only (Detector) 67480-3-PBS (5B1G3). 100 μg. Concentration 1 mgl/ml.

Alternative SOD1 matched antibody pairs: MP00799-1, MP00799-2, MP00799-3, MP50052-2

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 67480-2-PBS
Tested Reactivity human
Immunogen SOD1 fusion protein Ag28553
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=5.45 x 10-9M
KOff=2.92 x 10-4M
KOn=5.36 x 104M
Full Name superoxide dismutase 1, soluble
Synonyms IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1
GenBank accession numberBC001034
Gene symbol SOD1
UniprotP00441
Gene ID (NCBI) 6647
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 67480-3-PBS
Tested Reactivity human
Immunogen SOD1 fusion protein Ag28553
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=1.81 x 10-10M
KOff=3.56 x 10-6M
KOn=1.97 x 104M
Full Name superoxide dismutase 1, soluble
Synonyms IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1
GenBank accession numberBC001034
Gene symbol SOD1
UniprotP00441
Gene ID (NCBI) 6647
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C