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SON Polyclonal antibody

SON Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28046-1-AP

Print datasheet

Synonyms

SON3, SON DNA binding protein, KIAA1019, HSPC312, DBP5

验证数据展示

  • IHC staining of mouse colon using 28046-1-AP

    IHC staining of mouse colon using 28046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28046-1-AP (SON antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SON antibody included
  • IHC staining of human thyroid cancer using 28046-1-AP

    IHC staining of human thyroid cancer using 28046-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28046-1-AP (SON antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SON antibody included
  • IHC staining of human thyroid cancer using 28046-1-AP

    IHC staining of human thyroid cancer using 28046-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28046-1-AP (SON antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SON antibody included

经过测试的应用

Positive IHC detected inhuman thyroid cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SON Ready-To-Use IHC Kit. SON primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28046-1-AP targets SON in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SON fusion protein Ag27620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SON DNA binding protein
Synonyms SON3, SON DNA binding protein, KIAA1019, HSPC312, DBP5
Calculated Molecular Weight 264 aa
GenBank Accession NumberNM_001291411
Gene Symbol SON
Gene ID (NCBI) 6651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18583
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SON, also called C21orf50 and DBP5, is a nuclear speckle-localized protein that shares homology with pre-mRNA splicing accessory factors (PMID: 10950926). It is an RNA-binding protein that acts as an mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites, and specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG (PMID: 20581448; 21504830).

实验方案

Product Specific Protocols
IHC protocol for SON antibody 28046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols