• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83787-5-RR

    WB analysis using 83787-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83787-5-RR (SON antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 83787-5-RR

    IHC staining of human colon using 83787-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83787-5-RR (SON antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SON antibody included
  • IF Staining of U2OS using 83787-5-RR

    IF Staining of U2OS using 83787-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SON antibody (83787-5-RR, Clone: 240845B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U2OS using 83787-5-RR

    IF Staining of U2OS using 83787-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SON antibody (83787-5-RR, Clone: 240845B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83787-5-RR

    FC experiment of HeLa using 83787-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SON Recombinant antibody (83787-5-RR, Clone:240845B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83787-5-RR

    Affinity and Kinetic Characterization of 83787-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83787-5-RR against Human SON were performed. The affinity constant is 0.591 nM.

SON Recombinant antibody

SON Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83787-5-RR

产品说明书

CloneNo. 240845B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

SON DNA binding protein, KIAA1019, HSPC312, DBP5, C21orf50

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, human testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy SON Ready-To-Use IHC Kit. SON primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83787-5-RR targets SON in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SON fusion protein Ag27620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SON DNA binding protein
别名 SON DNA binding protein, KIAA1019, HSPC312, DBP5, C21orf50
计算分子量 264 aa
观测分子量 250-300 kDa
GenBank蛋白编号NM_001291411
基因名称 SON
Gene ID (NCBI) 6651
RRIDAB_3671379
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP18583
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SON, also called C21orf50 and DBP5, is a nuclear speckle-localized protein that shares homology with pre-mRNA splicing accessory factors (PMID: 10950926). It is an RNA-binding protein that acts as an mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites, and specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG (PMID: 20581448; 21504830).

实验方案

Product Specific Protocols
WB protocol for SON antibody 83787-5-RRDownload protocol
IHC protocol for SON antibody 83787-5-RRDownload protocol
IF protocol for SON antibody 83787-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols