验证数据展示
经过测试的应用
| Positive WB detected in | 3T3-L1 cells, HEK-293 cells, mouse kidney tissue, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human stomach tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13854-1-AP targets SORBS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 | SORBS1 fusion protein Ag4828 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorbin and SH3 domain containing 1 |
| 别名 | Sorbin and SH3 domain-containing protein 1, SORB1, SH3D5, c-Cbl-associated protein, CAP |
| 计算分子量 | 1292 aa, 142 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC042612 |
| 基因名称 | SORBS1 |
| Gene ID (NCBI) | 10580 |
| RRID | AB_11232224 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BX66 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SORBS1 antibody 13854-1-AP | Download protocol |
| IHC protocol for SORBS1 antibody 13854-1-AP | Download protocol |
| IP protocol for SORBS1 antibody 13854-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.
| ||
Front Cell Neurosci Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1. | ||
PLoS One Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells. | ||
Sci Rep STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl and regulates adipocyte differentiation | ||
PLoS One Proteomic changes in the hippocampus of large mammals after total-body low dose radiation |
