验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human kidney tissue, rat liver tissue, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IF | See 1 publications below |
产品信息
15881-1-AP targets Sorbitol dehydrogenase in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | Sorbitol dehydrogenase fusion protein Ag8651 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorbitol dehydrogenase |
| 别名 | SORD, EC:1.1.1.9, EC:1.1.1.56, EC:1.1.1.4, EC:1.1.1.14 |
| 计算分子量 | 357 aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC021085 |
| 基因名称 | SORD |
| Gene ID (NCBI) | 6652 |
| RRID | AB_2192275 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00796 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Sorbitol dehydrogenase antibody 15881-1-AP | Download protocol |
| IHC protocol for Sorbitol dehydrogenase antibody 15881-1-AP | Download protocol |
| IP protocol for Sorbitol dehydrogenase antibody 15881-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Endogenous fructose production and metabolism in the liver contributes to the development of metabolic syndrome. | ||
Reproduction Nicotine reduces Sord expression and tyrosine phosphorylation in capacitated sperm. | ||
J Mol Med (Berl) Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice. | ||
Chem Biol Interact Hepatocyte ferroptosis contributes to anti-tuberculosis drug-induced liver injury: Involvement of the HIF-1α/SLC7A11/GPx4 axis | ||
Nutrients Endogenous Fructose Production and Metabolism Drive Metabolic Dysregulation and Liver Disease in Mice with Hereditary Fructose Intolerance | ||
J Hepatol Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation |
