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  • Featured Product
  • KD/KO Validated

SORLA Polyclonal antibody

SORLA Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22592-1-AP

Print datasheet

Synonyms

SORL1, SorLA 1, SORL 1, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LDLR relative with 11 ligand-binding repeats

验证数据展示

  • WB analysis of mouse brain using 22592-1-AP

    WB analysis of mouse brain using 22592-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22592-1-AP (SORLA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 22592-1-AP

    IP experiment of mouse brain using 22592-1-AP

    IP result of anti-SORLA (IP:22592-1-AP, 5ug; Detection:22592-1-AP 1:500) with mouse brain tissue lysate 10000ug.

  • IHC staining of mouse brain using 22592-1-AP

    IHC staining of mouse brain using 22592-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22592-1-AP (SORLA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORL1 antibody included
  • IHC staining of mouse brain using 22592-1-AP

    IHC staining of mouse brain using 22592-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22592-1-AP (SORLA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORL1 antibody included
  • IF Staining of mouse cerebellum using 22592-1-AP

    IF Staining of mouse cerebellum using 22592-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SORLA antibody (22592-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy SORL1 Ready-To-Use IHC Kit. SORL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22592-1-AP targets SORLA in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen SORLA fusion protein Ag18445 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sortilin-related receptor, L(DLR class) A repeats-containing
Synonyms SORL1, SorLA 1, SORL 1, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LDLR relative with 11 ligand-binding repeats
Calculated Molecular Weight 2214 aa, 248 kDa
Observed Molecular Weight 300 kDa
GenBank Accession NumberBC137171
Gene Symbol SORLA
Gene ID (NCBI) 6653
RRIDAB_2879131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ92673
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SorLA also known as SorL1 or LR11, is a multiligand receptor belonging to the LDL receptor superfamily. It is also a member of vacuolar protein sorting-10 (Vps10) family and is enriched in the brain. SorLA acts as an sorting receptor for APP and prevents amyloidogenic processing of APP. Downregulation of SorLA has been found in patients with sporadic Alzheimer disease (AD). Currently SorLA has emerged as a potential target for AD therapy. (25404298)

实验方案

Product Specific Protocols
WB protocol for SORLA antibody 22592-1-APDownload protocol
IHC protocol for SORLA antibody 22592-1-APDownload protocol
IF protocol for SORLA antibody 22592-1-APDownload protocol
IP protocol for SORLA antibody 22592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB, IP

Proc Natl Acad Sci U S A

α2A adrenergic receptor promotes amyloidogenesis through disrupting APP-SorLA interaction.

Authors - Yunjia Chen
View Article
mouseWB

Environ Res

Exposure to a real traffic environment impairs brain cognition in aged mice

Authors - Chenlu Xu
View Article
humanWB,IHC,IF,CoIP

Funct Integr Genomics

SORL1 stabilizes ABCB1 to promote cisplatin resistance in ovarian cancer

Authors - Zhen Zheng
  • KD Validated
View Article
ratWB,IF

Adv Sci (Weinh)

Endo-Lysosomal Network Disorder Reprograms Energy Metabolism in SorL1-Null Rat Hippocampus

Authors - Yajie Wang
View Article