验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, K-562 cells, NIH/3T3 cells, THP-1 cells, mouse brain tissue, rat brain tissue |
Planning an IHC experiment? We recommend our IHCeasy SOS1 Ready-To-Use IHC Kit. SOS1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82784-2-RR targets SOS1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | son of sevenless homolog 1 (Drosophila) |
| 别名 | |
| 计算分子量 | 152 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | NM_005633 |
| 基因名称 | SOS1 |
| Gene ID (NCBI) | 6654 |
| RRID | AB_3670550 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q07889 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOS1 antibody 82784-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |