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SOSTDC1 Polyclonal antibody

SOSTDC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22213-1-AP

Print datasheet

Synonyms

CDA019, Ectodermal BMP inhibitor, ECTODIN, sclerostin domain containing 1, SOSTDC1, USAG 1, USAG1

验证数据展示

  • WB analysis using 22213-1-AP

    WB analysis using 22213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22213-1-AP (SOSTDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of Saos-2 using 22213-1-AP

    IF Staining of Saos-2 using 22213-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using SOSTDC1 antibody (22213-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman milk
Positive IF/ICC detected inSaos-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22213-1-AP targets SOSTDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SOSTDC1 fusion protein Ag17538 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sclerostin domain containing 1
Synonyms CDA019, Ectodermal BMP inhibitor, ECTODIN, sclerostin domain containing 1, SOSTDC1, USAG 1, USAG1
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberBC008484
Gene Symbol SOSTDC1
Gene ID (NCBI) 25928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6X4U4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOSTDC1 (Sclerostin domain-containing protein 1), also known as Ectodin (Ectodermal BMP inhibitor), is a member of the sclerostin family. It is an N-glycosylated, secreted protein with a C-terminal cystine knot-like domain and is highly abundant in milk and kidney. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically, it directly associates with BMPs, prohibiting them from binding their receptors, thereby regulating BMP signaling during cellular proliferation, differentiation, and programmed cell death.

实验方案

Product Specific Protocols
WB protocol for SOSTDC1 antibody 22213-1-APDownload protocol
IF protocol for SOSTDC1 antibody 22213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols