验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, mouse small intestine tissue, SH-SY5Y cells |
Positive IP detected in | mouse colon tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-Fro detected in | mouse brain tissue |
Positive FC (Intra) detected in | C6 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10422-1-AP targets SOX10 in WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF-Fro, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | SOX10 fusion protein Ag0673 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | SRY (sex determining region Y)-box 10 |
别名 | DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10 |
计算分子量 | 50 kDa |
观测分子量 | 50-60 kDa |
GenBank蛋白编号 | BC002824 |
基因名称 | SOX10 |
Gene ID (NCBI) | 6663 |
RRID | AB_2195371 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P56693 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)
实验方案
Product Specific Protocols | |
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WB protocol for SOX10 antibody 10422-1-AP | Download protocol |
IHC protocol for SOX10 antibody 10422-1-AP | Download protocol |
IF protocol for SOX10 antibody 10422-1-AP | Download protocol |
IP protocol for SOX10 antibody 10422-1-AP | Download protocol |
FC protocol for SOX10 antibody 10422-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Neuron Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson's and Alzheimer's disease co-pathology and behavior impairments | ||
Dev Cell Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination | ||
Front Immunol Machine learning-based identification of SOX10 as an immune regulator of macrophage in gliomas | ||
J Cereb Blood Flow Metab The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia | ||
Sci Rep Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models |