SOX10 Polyclonal antibody

SOX10 Polyclonal Antibody for WB, IHC, IF-Fro, FC (Intra), IP, ELISA
Cat No. 10422-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-Fro, FC (Intra), IP, ELISA and More (2)

DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, mouse small intestine tissue, SH-SY5Y cells
Positive IP detected inmouse colon tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse brain tissue
Positive FC (Intra) detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10422-1-AP targets SOX10 in WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 SOX10 fusion protein Ag0673 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 10
别名 DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10
计算分子量 50 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC002824
基因名称 SOX10
Gene ID (NCBI) 6663
RRIDAB_2195371
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56693
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

实验方案

Product Specific Protocols
WB protocol for SOX10 antibody 10422-1-APDownload protocol
IHC protocol for SOX10 antibody 10422-1-APDownload protocol
IF protocol for SOX10 antibody 10422-1-APDownload protocol
IP protocol for SOX10 antibody 10422-1-APDownload protocol
FC protocol for SOX10 antibody 10422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Regulation of intestinal immunity and tissue repair by enteric glia

Authors - Fränze Progatzky
mouseIF

Neuron

Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson's and Alzheimer's disease co-pathology and behavior impairments

Authors - Jie Xiang
mouse,ratWB,IF,CoIP

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
humanIF

Front Immunol

Machine learning-based identification of SOX10 as an immune regulator of macrophage in gliomas

Authors - Gelei Xiao
mouseWB

J Cereb Blood Flow Metab

The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia

Authors - Celeste Yin-Chieh Wu
ratIF

Sci Rep

Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models

Authors - Hiroaki Takeya
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