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SOX15 Polyclonal antibody

SOX15 Polyclonal Antibody for IF/ICC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, IHC,ELISA

Conjugate

Unconjugated

Cat No : 16725-1-AP

Print datasheet

Synonyms

Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27

验证数据展示

  • IHC staining of mouse embryo using 16725-1-AP

    IHC staining of mouse embryo using 16725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 16725-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOX15 antibody included
  • IF Staining of mouse embryo using 16725-1-AP

    IF Staining of mouse embryo using 16725-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 16725-1-AP (SOX15 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse embryo tissue
Planning an IHC experiment? We recommend our IHCeasy SOX15 Ready-To-Use IHC Kit. SOX15 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 16725-1-AP is SOX15 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC,ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Immunogen SOX15 fusion protein Ag10173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 15
Synonyms Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC000985
Gene Symbol SOX15
Gene ID (NCBI) 6665
RRIDAB_2878305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60248
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SOX15 antibody 16725-1-APDownload protocol
IF protocol for SOX15 antibody 16725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,pigIF

Cell Rep

Integration of single-cell transcriptome and chromatin accessibility of early gonads development among goats, pigs, macaques, and humans

Authors - Min Chen
View Article