• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SOX2 Recombinant antibody, PBS Only

SOX2 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230225G1

Cat no : 83059-5-PBS

Print datasheet

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

验证数据展示

  • IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red). This data was developed using the same antibody clone with 83059-5-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83059-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83059-5-RR

    Affinity and Kinetic Characterization of 83059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83059-5-RR against Human SOX2 were performed. The affinity constant is 3.54 nM.

Planning an IHC experiment? We recommend our IHCeasy SOX2 Ready-To-Use IHC Kit. SOX2 primary antibody included.

产品信息

83059-5-PBS targets SOX2 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SOX2 fusion protein Ag0612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SRY (sex determining region Y)-box 2
Synonyms Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3
Calculated Molecular Weight 34 kDa
GenBank Accession NumberBC013923
Gene Symbol SOX2
Gene ID (NCBI) 6657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undiferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal diferentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ) , a well-known stem cell niche of the adult brain.