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验证数据展示

  • IF Staining of U-251 using 83059-5-RR

    IF Staining of U-251 using 83059-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83059-5-RR

    Affinity and Kinetic Characterization of 83059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83059-5-RR against Human SOX2 were performed. The affinity constant is 3.54 nM.

SOX2 Recombinant antibody

SOX2 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83059-5-RR

产品说明书

CloneNo. 230225G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy SOX2 Ready-To-Use IHC Kit. SOX2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83059-5-RR targets SOX2 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SOX2 fusion protein Ag0612 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 2
别名 Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3
计算分子量 34 kDa
GenBank蛋白编号BC013923
基因名称 SOX2
Gene ID (NCBI) 6657
RRIDAB_3670787
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undiferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal diferentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ) , a well-known stem cell niche of the adult brain.

实验方案

Product Specific Protocols
IF protocol for SOX2 antibody 83059-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols