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SOX4 Recombinant antibody

SOX4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240625E3

Cat no : 83660-1-RR

Print datasheet

Synonyms

Transcription factor SOX-4, EVI16, CSS10, 240625E3

验证数据展示

  • FC experiment of SH-SY5Y using 83660-1-RR

    FC experiment of SH-SY5Y using 83660-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • WB analysis using 83660-1-RR

    WB analysis using 83660-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83660-1-RR

    Affinity and Kinetic Characterization of 83660-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, A431 cells, MCF-7 cells, SGC-7901 cells
Positive FC (Intra) detected inSH-SY5Y cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83660-1-RR targets SOX4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SOX4 fusion protein Ag26583 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SRY (sex determining region Y)-box 4
Synonyms Transcription factor SOX-4, EVI16, CSS10, 240625E3
Calculated Molecular Weight 474 aa, 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC072668
Gene Symbol SOX4
Gene ID (NCBI) 6659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa.

实验方案

Product Specific Protocols
WB protocol for SOX4 antibody 83660-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols