验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, SH-SY5Y cells, U-87 MG cells, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13216-1-AP targets SOX5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SOX5 fusion protein Ag4092 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 5 |
| 别名 | L SOX5, SOX5, Transcription factor SOX 5 |
| 计算分子量 | 763 aa, 84 kDa |
| 观测分子量 | 68-70 kDa |
| GenBank蛋白编号 | BC029220 |
| 基因名称 | SOX5 |
| Gene ID (NCBI) | 6660 |
| RRID | AB_2196089 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35711 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sex determining region Y-box protein 5 (SOX5) is a member of the SOX family, which was identified based on the conserved homology of the high-mobility group DNA-binding motif. It has been reported that SOX5 is involved in the regulation of embryonic development, and is associated with various cancer types, including prostate cancer, glioblastoma, hepatocellular carcinoma, osteosarcoma and nasopharyngeal carcinoma. SOX5 has 5 isoforms with the molecular mass of 42, 71 and 82-84 kDa. (PMID: 30854049)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX5 antibody 13216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination. | ||
Pharmacol Res Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways. | ||
Cell Oncol (Dordr) Apatinib inhibits glycolysis by suppressing the VEGFR2/AKT1/SOX5/GLUT4 signaling pathway in ovarian cancer cells.
| ||
Phytomedicine Transcriptomics, molecular docking, and cross-resistance profiling of nobiletin in cancer cells and synergistic interaction with doxorubicin upon SOX5 transfection.
| ||
Lab Invest Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1. | ||
Mol Cell Biochem Expression profiles of Sox transcription factors within the postnatal rodent testes. |