验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, mouse heart tissue, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23925-1-AP targets SOX7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SOX7 fusion protein Ag10323 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 7 |
| 别名 | Transcription factor SOX-7, Transcription factor SOX 7, SOX 7, endoderm lineage markers |
| 计算分子量 | 388 aa, 42 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC071947 |
| 基因名称 | SOX7 |
| Gene ID (NCBI) | 83595 |
| RRID | AB_2879363 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BT81 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOX7, also named as Transcription factor SOX-7, is a 388 amino acid protein, which is widely expressed in adult and fetal tissues and tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation. SOX7 binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. SOX7 may be required for the survival of both hematopoietic and endothelial precursors during specification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX7 antibody 23925-1-AP | Download protocol |
| IHC protocol for SOX7 antibody 23925-1-AP | Download protocol |
| IF protocol for SOX7 antibody 23925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FASEB J System analysis based on weighted gene co-expression analysis identifies SOX7 as a novel regulator of hepatic stellate cell activation and liver fibrosis | ||
Mol Genet Genomics Predisposition to atrioventricular septal defects may be caused by SOX7 variants that impair interaction with GATA4. | ||
Cell Death Dis The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling. | ||
Int J Biol Macromol Integrated transcriptomics and lipidomics reveals protective effect in vascular endothelial barrier of a polysaccharide from Typhae Pollen |
