验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, COLO 320 cells, rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20627-1-AP targets SOX8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SOX8 fusion protein Ag14556 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 8 |
| 别名 | SOX8, Transcription factor SOX 8 |
| 计算分子量 | 446 aa, 47 kDa |
| 观测分子量 | 47-55 kDa |
| GenBank蛋白编号 | BC031797 |
| 基因名称 | SOX8 |
| Gene ID (NCBI) | 30812 |
| RRID | AB_10693538 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P57073 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX8 antibody 20627-1-AP | Download protocol |
| IHC protocol for SOX8 antibody 20627-1-AP | Download protocol |
| IF protocol for SOX8 antibody 20627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Thymic epithelial cells co-opt lineage-defining transcription factors to eliminate autoreactive T cells. | ||
Carcinogenesis SOX8 acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer. | ||
Redox Biol Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis
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