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  • Featured Product
  • KD/KO Validated

SOX8 Polyclonal antibody

SOX8 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 20627-1-AP

Print datasheet

Synonyms

SOX8, Transcription factor SOX 8

验证数据展示

  • WB analysis using 20627-1-AP

    WB analysis using 20627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20627-1-AP (SOX8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20627-1-AP

    IHC staining of human brain using 20627-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20627-1-AP (SOX8 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HeLa using 20627-1-AP

    IF Staining of HeLa using 20627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20627-1-AP (SOX8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, COLO 320 cells, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20627-1-AP targets SOX8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SOX8 fusion protein Ag14556 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 8
Synonyms SOX8, Transcription factor SOX 8
Calculated Molecular Weight 446 aa, 47 kDa
Observed Molecular Weight 47-55 kDa
GenBank Accession NumberBC031797
Gene Symbol SOX8
Gene ID (NCBI) 30812
RRIDAB_10693538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57073
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SOX8 antibody 20627-1-APDownload protocol
IHC protocol for SOX8 antibody 20627-1-APDownload protocol
IF protocol for SOX8 antibody 20627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

Thymic epithelial cells co-opt lineage-defining transcription factors to eliminate autoreactive T cells.

Authors - Daniel A Michelson
View Article
humanIHC

Cancer Biol Ther

The oncogenic role of SOX8 in endometrial carcinoma.

Authors - Wenyan Tian
View Article
humanWB,IHC

Carcinogenesis

SOX8 acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer.

Authors - Hailin Tang
View Article
humanWB,IHC

Redox Biol

Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis

Authors - Xue Yang
  • KO Validated
View Article