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验证数据展示

  • WB analysis using 66508-1-Ig (same clone as 66508-1-PBS)

    WB analysis using 66508-1-Ig (same clone as 66508-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    IHC staining of human lung cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SP1 antibody included
  • IF Staining of human colon cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    IF Staining of human colon cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    IF Staining of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    FC experiment of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SP1 (66508-1-Ig, Clone:4G3H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

SP1 Monoclonal antibody, PBS Only

SP1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 66508-1-PBS

产品说明书

CloneNo. 4G3H3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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66508-1-Ig

Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.

产品信息

66508-1-PBS targets SP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SP1 fusion protein Ag16720 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Sp1 transcription factor
别名 TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3
计算分子量 785 aa, 81 kDa
观测分子量 95-105 kDa, 60-65 kDa
GenBank蛋白编号BC062539
基因名称 SP1
Gene ID (NCBI) 6667
ENSEMBL Gene IDENSG00000185591
RRIDAB_2881871
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08047
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].