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SP1 Monoclonal antibody, PBS Only

SP1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4G3H3

Cat no : 66508-1-PBS

Print datasheet

Synonyms

TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3

验证数据展示

  • WB analysis using 66508-1-Ig (same clone as 66508-1-PBS)

    WB analysis using 66508-1-Ig (same clone as 66508-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    IHC staining of human lung cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SP1 antibody included
  • IF Staining of human colon cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    IF Staining of human colon cancer using 66508-1-Ig (same clone as 66508-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    IF Staining of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    FC experiment of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SP1 (66508-1-Ig, Clone:4G3H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

66508-1-Ig

Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.

产品信息

66508-1-PBS targets SP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SP1 fusion protein Ag16720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Sp1 transcription factor
Synonyms TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3
Calculated Molecular Weight 785 aa, 81 kDa
Observed Molecular Weight 95-105 kDa, 60-65 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
ENSEMBL Gene IDENSG00000185591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08047
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].