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SP100 Polyclonal antibody

SP100 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31102-1-AP

Print datasheet

Synonyms

DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa

验证数据展示

  • WB analysis using 31102-1-AP

    WB analysis using 31102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31102-1-AP (SP100 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31102-1-AP targets SP100 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen SP100 fusion protein Ag35193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SP100 nuclear antigen
Synonyms DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa
Calculated Molecular Weight 879 aa, 100 kDa
Observed Molecular Weight75-85 kDa
GenBank Accession NumberBC011562
Gene Symbol SP100
Gene ID (NCBI) 6672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP23497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains.

实验方案

Product Specific Protocols
WB protocol for SP100 antibody 31102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols