验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31102-1-AP targets SP100 in WB, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human |
免疫原 | SP100 fusion protein Ag35193 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | SP100 nuclear antigen |
别名 | DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa |
计算分子量 | 879 aa, 100 kDa |
观测分子量 | 75-85 kDa |
GenBank蛋白编号 | BC011562 |
基因名称 | SP100 |
Gene ID (NCBI) | 6672 |
RRID | AB_3669856 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | P23497 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains.
实验方案
Product Specific Protocols | |
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WB protocol for SP100 antibody 31102-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |