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验证数据展示

  • WB analysis using 84012-4-RR (same clone as 84012-4-PBS)

    WB analysis using 84012-4-RR (same clone as 84012-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84012-4-RR (SP100 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84012-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00964-1

    Cytometric bead array standard curve of MP00964-1

    Cytometric bead array standard curve of MP00964-1, SP100 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84012-4-PBS. Detection antibody: 84012-2-PBS. Standard: Ag35193. Range: 0.156-10 ng/mL.

  • Affinity and Kinetic Characterization of 84012-4-RR

    Affinity and Kinetic Characterization of 84012-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84012-4-RR against Human SP100 were performed. The affinity constant is 0.127 nM.

SP100 Recombinant antibody, PBS Only (Capture)

SP100 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84012-4-PBS

产品说明书

CloneNo. 241153F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.

产品信息

84012-4-PBS targets SP100 as part of a matched antibody pair:

MP00964-1: 84012-4-PBS capture and 84012-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SP100 fusion protein Ag35193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SP100 nuclear antigen
别名 Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100
计算分子量 879 aa, 100 kDa
观测分子量70-100 kDa
GenBank蛋白编号BC011562
基因名称 SP100
Gene ID (NCBI) 6672
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23497
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.