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SP100 Recombinant antibody

SP100 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241153F8

Cat no : 84012-5-RR

Print datasheet

Synonyms

Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100

验证数据展示

  • WB analysis using 84012-5-RR

    WB analysis using 84012-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84012-5-RR (SP100 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84012-5-RR

    IF Staining of A431 using 84012-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 84012-5-RR

    IF Staining of A431 using 84012-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84012-5-RR is SP100 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SP100 fusion protein Ag35193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SP100 nuclear antigen
Synonyms Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100
Calculated Molecular Weight 879 aa, 100 kDa
Observed Molecular Weight70-100 kDa, 110-140 kDa
GenBank Accession NumberBC011562
Gene Symbol SP100
Gene ID (NCBI) 6672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.

实验方案

Product Specific Protocols
WB protocol for SP100 antibody 84012-5-RRDownload protocol
IF protocol for SP100 antibody 84012-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols