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SP110 Polyclonal antibody

SP110 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11502-1-AP

Print datasheet

Synonyms

Speckled 110 kDa, SP110 nuclear body protein, IPR1, Interferon-induced protein 41/75, IFI75

验证数据展示

  • WB analysis of HeLa using 11502-1-AP

    WB analysis of HeLa using 11502-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 11502-1-AP

    WB analysis using 11502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 11502-1-AP

    FC experiment of Jurkat using 11502-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SP110 (11502-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IP experiment of HeLa using 11502-1-AP

    IP experiment of HeLa using 11502-1-AP

    IP result of anti-SP110 (IP:11502-1-AP, 4ug; Detection:11502-1-AP 1:800) with HeLa cells lysate 4000ug.

经过测试的应用

Positive WB detected inA2780 cells, PC-3 cells, human brain tissue, Jurkat cells, K-562 cells, human testis tissue, HeLa cells, COLO 320 cells.
Positive IP detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11502-1-AP targets SP110 in WB, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SP110 fusion protein Ag2042 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SP110 nuclear body protein
Synonyms Speckled 110 kDa, SP110 nuclear body protein, IPR1, Interferon-induced protein 41/75, IFI75
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight 62-66 kDa
GenBank Accession NumberBC019059
Gene Symbol SP110
Gene ID (NCBI) 3431
RRIDAB_10863116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB58
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SP110 antibody 11502-1-APDownload protocol
IP protocol for SP110 antibody 11502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,FC

J Clin Immunol

Detection of Sp110 by Flow Cytometry and Application to Screening Patients for Veno-occlusive Disease with Immunodeficiency.

Authors - Florian A Marquardsen
View Article