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SP2 Polyclonal antibody

SP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25000-1-AP

Print datasheet

Synonyms

KIAA0048, SP2, Sp2 transcription factor, Transcription factor Sp2

验证数据展示

  • WB analysis of Jurkat using 25000-1-AP

    WB analysis of Jurkat using 25000-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 25000-1-AP

    WB analysis of K-562 using 25000-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, K-562 cells, C6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

25000-1-AP targets SP2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen SP2 fusion protein Ag8060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sp2 transcription factor
Synonyms KIAA0048, SP2, Sp2 transcription factor, Transcription factor Sp2
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC016680
Gene Symbol SP2
Gene ID (NCBI) 6668
RRIDAB_2879839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP2 also named as Sp2 transcription factor or KIAA0048 is a 613 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the sp1 C2H2-type zinc finger protein family. SP2 binds to box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites in nucleus . SP2 may regulate expression of the T-cell antigen receptor (TCR) gene.

实验方案

Product Specific Protocols
WB protocol for SP2 antibody 25000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Commun Biol

ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer

Authors - Rebecca Garnham
View Article