SP3 Polyclonal antibody
SP3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, C2C12 cell, COLO 320 cells |
Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
IHC | See 1 publications below |
产品信息
26584-1-AP targets SP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | SP3 fusion protein Ag25082 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Sp3 transcription factor |
Synonyms | SP3, Sp3 transcription factor, SPR 2, Transcription factor Sp3 |
Calculated Molecular Weight | 781 aa, 82 kDa |
Observed Molecular Weight | 70 kDa, 115 kDa |
GenBank Accession Number | BC126414 |
Gene Symbol | SP3 |
Gene ID (NCBI) | 6670 |
RRID | AB_2880563 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q02447 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SP3 antibody 26584-1-AP | Download protocol |
IHC protocol for SP3 antibody 26584-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Dig Dis Sci Speckle-Type POZ Protein Down-Regulates Matrix Metalloproteinase 2 Expression via Sp1/PI3K/Akt Signaling Pathway in Colorectal Cancer. | ||
Biochem Biophys Res Commun Transcriptional activation of CSTB gene expression by transcription factor Sp3 | ||
Mol Cell Probes LINC00115 regulates lung adenocarcinoma progression via sponging miR-154-3p to modulate Sp3 expression | ||
Cell Rep LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis | ||
Int Immunopharmacol Menstrual blood-derived stem cells exosomal miR-let-7 to ameliorate pulmonary fibrosis through inhibiting ferroptosis by Sp3/HDAC2/Nrf2 signaling pathway |