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SP6 Polyclonal antibody

SP6 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21234-1-AP

Print datasheet

Synonyms

EPFN, EPIPROFIN, SP6, Sp6 transcription factor, Transcription factor Sp6

验证数据展示

  • WB analysis of fetal human brain using 21234-1-AP

    WB analysis of fetal human brain using 21234-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 21234-1-AP

    IHC staining of human skin using 21234-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 21234-1-AP

    IHC staining of human skin using 21234-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of Caco-2 using 21234-1-AP

    IF Staining of Caco-2 using 21234-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SP6 antibody (21234-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected infetal human brain tissue, mouse brain tissue
Positive IHC detected inhuman skin tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21234-1-AP targets SP6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SP6 fusion protein Ag15707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sp6 transcription factor
Synonyms EPFN, EPIPROFIN, SP6, Sp6 transcription factor, Transcription factor Sp6
Calculated Molecular Weight 376 aa, 40 kDa
GenBank Accession NumberBC103951
Gene Symbol SP6
Gene ID (NCBI) 80320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SY56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP6 (chromosome 17) is a 376 amino acid protein, which localizes in nucleus. SP6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. The levels of sp6 protein expressed in various tissues in the embryo. This antibody detects the SP6 protein. It can't detect the KLF14/BTEB5.

This antibody was previously listed as a KLF14 antibody, because the SP6 gene on chromosome 17q21.32 has been referred to as KLF14 in the literature. SP6 should not be confused with the KLF14 gene on chromosome 7q32.2.

实验方案

Product Specific Protocols
WB protocol for SP6 antibody 21234-1-APDownload protocol
IHC protocol for SP6 antibody 21234-1-APDownload protocol
IF protocol for SP6 antibody 21234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Int J Biol Sci

The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer.

Authors - Guiyang Wu
View Article