验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue |
| Positive IHC detected in | human skin tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
21234-1-AP targets SP6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SP6 fusion protein Ag15707 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Sp6 transcription factor |
| 别名 | EPFN, EPIPROFIN, SP6, Sp6 transcription factor, Transcription factor Sp6 |
| 计算分子量 | 376 aa, 40 kDa |
| GenBank蛋白编号 | BC103951 |
| 基因名称 | SP6 |
| Gene ID (NCBI) | 80320 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3SY56 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SP6 (chromosome 17) is a 376 amino acid protein, which localizes in nucleus. SP6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. The levels of sp6 protein expressed in various tissues in the embryo. This antibody detects the SP6 protein. It can't detect the KLF14/BTEB5.
This antibody was previously listed as a KLF14 antibody, because the SP6 gene on chromosome 17q21.32 has been referred to as KLF14 in the literature. SP6 should not be confused with the KLF14 gene on chromosome 7q32.2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SP6 antibody 21234-1-AP | Download protocol |
| IHC protocol for SP6 antibody 21234-1-AP | Download protocol |
| IF protocol for SP6 antibody 21234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
