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验证数据展示

  • WB analysis using 28561-1-AP

    WB analysis using 28561-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28561-1-AP (SP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 28561-1-AP

    IHC staining of human stomach cancer using 28561-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28561-1-AP (SP6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28561-1-AP

    IF Staining of HepG2 using 28561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SP6 antibody (28561-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

SP6 Polyclonal antibody

SP6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28561-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Krueppel-like factor 14, KLF14, EPIPROFIN, EPFN, AI1K

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28561-1-AP targets SP6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SP6 fusion protein Ag29372 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sp6 transcription factor
Synonyms Krueppel-like factor 14, KLF14, EPIPROFIN, EPFN, AI1K
Calculated Molecular Weight 376 aa, 40 kDa
Observed Molecular Weight40-44 kDa
GenBank Accession NumberBC103951
Gene Symbol SP6
Gene ID (NCBI) 80320
RRIDAB_3669661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SY56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SP6 antibody 28561-1-APDownload protocol
IHC protocol for SP6 antibody 28561-1-APDownload protocol
IF protocol for SP6 antibody 28561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols