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验证数据展示

  • WB analysis using 28561-1-AP

    WB analysis using 28561-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28561-1-AP (SP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 28561-1-AP

    IHC staining of human stomach cancer using 28561-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28561-1-AP (SP6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28561-1-AP

    IF Staining of HepG2 using 28561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SP6 antibody (28561-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

SP6 Polyclonal antibody

SP6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28561-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Krueppel-like factor 14, KLF14, EPIPROFIN, EPFN, AI1K

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28561-1-AP targets SP6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SP6 fusion protein Ag29372 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sp6 transcription factor
别名 Krueppel-like factor 14, KLF14, EPIPROFIN, EPFN, AI1K
计算分子量 376 aa, 40 kDa
观测分子量40-44 kDa
GenBank蛋白编号BC103951
基因名称 SP6
Gene ID (NCBI) 80320
RRIDAB_3669661
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SY56
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SP6 antibody 28561-1-APDownload protocol
IHC protocol for SP6 antibody 28561-1-APDownload protocol
IF protocol for SP6 antibody 28561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols