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SPACA1 Polyclonal antibody

SPACA1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12829-1-AP

Print datasheet

Synonyms

SAMP32, SPACA1, sperm acrosome associated 1

验证数据展示

  • IHC staining of human testis using 12829-1-AP

    IHC staining of human testis using 12829-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12829-1-AP

    IHC staining of human testis using 12829-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12829-1-AP targets SPACA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SPACA1 fusion protein Ag3549 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm acrosome associated 1
Synonyms SAMP32, SPACA1, sperm acrosome associated 1
Calculated Molecular Weight 294 aa, 32 kDa
GenBank Accession NumberBC029488
Gene Symbol SPACA1
Gene ID (NCBI) 81833
RRIDAB_2877886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPACA1, also named as SAMP32, is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm-egg fusion. SPACA1 is regulated by phosphorylation.

实验方案

Product Specific Protocols
IHC protocol for SPACA1 antibody 12829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Free Radic Res

Heterozygous SOD1 deficiency in mice with an NZW background causes male infertility and an aberrant immune phenotype

Authors - Takujiro Homma
View Article