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验证数据展示

  • WB analysis of HEK-293 using 21137-1-AP

    WB analysis of HEK-293 using 21137-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 21137-1-AP

    IF Staining of HEK-293 using 21137-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21137-1-AP (SPACA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SPACA3 Polyclonal antibody

SPACA3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21137-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17, Lysozyme like protein 3, LYC3, CT54, Cancer/testis antigen 54

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21137-1-AP targets SPACA3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPACA3 fusion protein Ag15589 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm acrosome associated 3
别名 Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17, Lysozyme like protein 3, LYC3, CT54, Cancer/testis antigen 54
计算分子量 215 aa, 23 kDa
观测分子量 28-32 kDa, 46-50 kDa
GenBank蛋白编号BC029867
基因名称 SPACA3
Gene ID (NCBI) 124912
RRIDAB_10913810
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IXA5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPACA3, also named as LYC3, LYZL3, SLLP1, SPRASA, CT54 and ALLP-17, belongs to the glycosyl hydrolase 22 family. It is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. SPACA3 has a Dimer form(PMID:15982649 ).

实验方案

Product Specific Protocols
WB protocol for SPACA3 antibody 21137-1-APDownload protocol
IF protocol for SPACA3 antibody 21137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols