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SPACA3 Polyclonal antibody

SPACA3 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21137-1-AP

Print datasheet

Synonyms

1700025M08Rik, ALLP17, Cancer/testis antigen 54, CT54, LYC3, Lysozyme like protein 3, LYZL3, SLLP1, SPACA3, sperm acrosome associated 3, Sperm lysozyme like protein 1, SPRASA

验证数据展示

  • WB analysis of HEK-293 using 21137-1-AP

    WB analysis of HEK-293 using 21137-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 21137-1-AP

    IF Staining of HEK-293 using 21137-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21137-1-AP (SPACA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21137-1-AP is SPACA3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SPACA3 fusion protein Ag15589 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm acrosome associated 3
Synonyms 1700025M08Rik, ALLP17, Cancer/testis antigen 54, CT54, LYC3, Lysozyme like protein 3, LYZL3, SLLP1, SPACA3, sperm acrosome associated 3, Sperm lysozyme like protein 1, SPRASA
Calculated Molecular Weight 215 aa, 23 kDa
Observed Molecular Weight 28-32 kDa, 46-50 kDa
GenBank Accession NumberBC029867
Gene Symbol SPACA3
Gene ID (NCBI) 124912
RRIDAB_10913810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXA5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPACA3, also named as LYC3, LYZL3, SLLP1, SPRASA, CT54 and ALLP-17, belongs to the glycosyl hydrolase 22 family. It is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. SPACA3 has a Dimer form(PMID:15982649 ).

实验方案

Product Specific Protocols
WB protocol for SPACA3 antibody 21137-1-APDownload protocol
IF protocol for SPACA3 antibody 21137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols