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验证数据展示

  • WB analysis using 84809-4-RR

    WB analysis using 84809-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84809-4-RR (SPAG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84809-4-RR

    Affinity and Kinetic Characterization of 84809-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84809-4-RR against Human SPAG5 were performed. The affinity constant is below 1 pM.

SPAG5 Recombinant antibody

SPAG5 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84809-4-RR

产品说明书

CloneNo. 242374H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest, Astrin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, PC-3 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84809-4-RR targets SPAG5 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SPAG5 fusion protein Ag6485 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sperm associated antigen 5
别名 Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest, Astrin
计算分子量 134 kDa
观测分子量 135 kDa, 150 kDa
GenBank蛋白编号BC000322
基因名称 SPAG5
Gene ID (NCBI) 10615
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96R06
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

实验方案

Product Specific Protocols
WB protocol for SPAG5 antibody 84809-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols