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SPAG9 Polyclonal antibody

SPAG9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24423-1-AP

Print datasheet

Synonyms

HLC-6, HLC4, HLC 6, CT89, C-Jun-amino-terminal kinase-interacting protein 4

验证数据展示

  • WB analysis using 24423-1-AP

    WB analysis using 24423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24423-1-AP

    WB analysis using 24423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24423-1-AP

    IHC staining of human testis using 24423-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 24423-1-AP

    IHC staining of human testis using 24423-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 24423-1-AP

    IF Staining of HeLa using 24423-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG9 antibody (24423-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IF Staining of RAW 264.7 using 24423-1-AP

    IF Staining of RAW 264.7 using 24423-1-AP

    Immunofluorescent analysis of RAW 264.7 cells using 24423-1-AP (SPAG9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, RAW 264.7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24423-1-AP targets SPAG9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SPAG9 fusion protein Ag19763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm associated antigen 9
Synonyms HLC-6, HLC4, HLC 6, CT89, C-Jun-amino-terminal kinase-interacting protein 4
Calculated Molecular Weight 1321 aa, 146 kDa
Observed Molecular Weight 146 kDa
GenBank Accession NumberBC146755
Gene Symbol SPAG9
Gene ID (NCBI) 9043
RRIDAB_2879541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60271
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPAG9 antibody 24423-1-APDownload protocol
IHC protocol for SPAG9 antibody 24423-1-APDownload protocol
IF protocol for SPAG9 antibody 24423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols