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SPAG9 Polyclonal antibody

SPAG9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24423-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 89, CT89, HLC 6, HLC4, HSS, JIP 4, JLP, JNK interacting protein 4, KIAA0516, MAPK8IP4, PHET, PIG6, SPAG9, sperm associated antigen 9, Sperm specific protein, Sperm surface protein, Sunday driver 1, SYD1

验证数据展示

  • WB analysis using 24423-1-AP

    WB analysis using 24423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24423-1-AP

    WB analysis using 24423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 24423-1-AP

    IF Staining of RAW 264.7 using 24423-1-AP

    Immunofluorescent analysis of RAW 264.7 cells using 24423-1-AP (SPAG9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human testis using 24423-1-AP

    IHC staining of human testis using 24423-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 24423-1-AP

    IHC staining of human testis using 24423-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, RAW 264.7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24423-1-AP targets SPAG9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SPAG9 fusion protein Ag19763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm associated antigen 9
Synonyms Cancer/testis antigen 89, CT89, HLC 6, HLC4, HSS, JIP 4, JLP, JNK interacting protein 4, KIAA0516, MAPK8IP4, PHET, PIG6, SPAG9, sperm associated antigen 9, Sperm specific protein, Sperm surface protein, Sunday driver 1, SYD1
Calculated Molecular Weight 1321 aa, 146 kDa
Observed Molecular Weight 146 kDa
GenBank Accession NumberBC146755
Gene Symbol SPAG9
Gene ID (NCBI) 9043
RRIDAB_2879541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60271
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPAG9 antibody 24423-1-APDownload protocol
IHC protocol for SPAG9 antibody 24423-1-APDownload protocol
IF protocol for SPAG9 antibody 24423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols