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验证数据展示

  • WB analysis of DU 145 using 19527-1-AP

    WB analysis of DU 145 using 19527-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 19527-1-AP

    IF Staining of HEK-293 using 19527-1-AP

    Immunofluorescent analysis of HEK-293 cells using 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human testis using 19527-1-AP

    IHC staining of human testis using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

  • IHC staining of human skin cancer using 19527-1-AP

    IHC staining of human skin cancer using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

SPANXA2/SPANXA1 Polyclonal antibody

SPANXA2/SPANXA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19527-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Cancer/testis antigen 11.1, CT11.1, SPAN X, SPANX, SPANX A, SPANX family member A, SPANX family, member A2, SPANXA, SPANXA1, SPANXA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, HEK-293 cells, PC-3 cells
Positive IHC detected inhuman skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19527-1-AP targets SPANXA2/SPANXA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SPANX family, member A2
别名 Cancer/testis antigen 11.1, CT11.1, SPAN X, SPANX, SPANX A, SPANX family member A, SPANX family, member A2, SPANXA, SPANXA1, SPANXA2
计算分子量 11 kDa
观测分子量 27 kDa
GenBank蛋白编号NM_145662
基因名称 SPANXA2/SPANXA1
Gene ID (NCBI) 728712
RRIDAB_2878586
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPANXA1, also named as SPANXA and SPANXA2, is a member of the SPANX family of cancer/testis-associated genes. It encodes differentially expressed testis-specific proteins that localize to various subcellular compartments. On Western blots of human testis proteins, SPANX antibodies reacts with the bands migrating between 15-30 kDa. The SPANX protein may be a useful target in cancer immunotherapy.

实验方案

Product Specific Protocols
WB protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
IHC protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
IF protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols