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SPANXA2/SPANXA1 Polyclonal antibody

SPANXA2/SPANXA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19527-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 11.1, CT11.1, SPAN X, SPANX, SPANX A, SPANX family member A, SPANX family, member A2, SPANXA, SPANXA1, SPANXA2

验证数据展示

  • WB analysis of DU 145 using 19527-1-AP

    WB analysis of DU 145 using 19527-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 19527-1-AP

    IF Staining of HEK-293 using 19527-1-AP

    Immunofluorescent analysis of HEK-293 cells using 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human testis using 19527-1-AP

    IHC staining of human testis using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

  • IHC staining of human skin cancer using 19527-1-AP

    IHC staining of human skin cancer using 19527-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

经过测试的应用

Positive WB detected inDU 145 cells, HEK-293 cells, PC-3 cells
Positive IHC detected inhuman skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19527-1-AP targets SPANXA2/SPANXA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SPANX family, member A2
Synonyms Cancer/testis antigen 11.1, CT11.1, SPAN X, SPANX, SPANX A, SPANX family member A, SPANX family, member A2, SPANXA, SPANXA1, SPANXA2
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberNM_145662
Gene Symbol SPANXA2/SPANXA1
Gene ID (NCBI) 728712
RRIDAB_2878586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NS26
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPANXA1, also named as SPANXA and SPANXA2, is a member of the SPANX family of cancer/testis-associated genes. It encodes differentially expressed testis-specific proteins that localize to various subcellular compartments. On Western blots of human testis proteins, SPANX antibodies reacts with the bands migrating between 15-30 kDa. The SPANX protein may be a useful target in cancer immunotherapy.

实验方案

Product Specific Protocols
WB protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
IHC protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
IF protocol for SPANXA2/SPANXA1 antibody 19527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols